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SDS-PAGE analysis of the expression of the wt6pgdh gene. (A) pET-trx-wt6pgdh in E. coli BL21(DE3), (B) pET-trx-wt6pgdh in E. coli BL21(DE3)-RIL, (C) pET-ci-wt6pgdh in E. coli BL21(DE3) and (D) pET-ci-wt6pgdh in E. coli BL21(DE3)-RIL. BI, before induction; T, total cellular proteins; S, soluble proteins; P, purified protein. Cells were grown at 37°C until A600 of 0.6. The induction condition was 20 μM IPTG at 20°C for 6 h.
