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SDS-PAGE analysis of the expression and purification of T. maritima 6PGDH from the E. coli BL21(DE3)/pET-co6pgdh-his (A) and E. coli BL21(DE3)/pET-co6pgdh (B). Cells were grown at 37°C until A600 of 0.6. The induction condition was 500 μM IPTG at 37°C for 4 h.
