Figure 8.

Production and purification of T. maritima 6PGDH. The 6PGDH amounts were calculated based on their activity relative to the purified 6PGDH specific activity. The amount of total T. maritima 6PGDH proteins produced by E. coli were estimated from the band intensities on the SDS-PAGE gels relative to those contained in the soluble fraction. Expression host was E. coli BL21(DE3) except where ** denoted E. coli BL21(DE3)-RIL. The data from left to right were obtained from Figure 1A, Figure 1C, Figure 1D, Figure 3A, Figure 3B, Figure 5A, and the expression by the E. coli BL21(DE3)/pET-co6pgdh (induced by 100 μM lactose at 37°C for 10 h).