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a) Total proteins of strains grown at 30°C extracted at 0, 2 h and 6 h after induction. b) Total [T], soluble [S] and aggregated [I] protein fractions extracted from recombinant strains 4 hours after IPTG addition. Arrows indicate the position of the recombinant protein. c) Growth profile of the three strains used in this study. After growth at 37°C, cultures were shifted to 30°C for 30 minutes prior to 0.1 mM IPTG addition.
