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. 2009 Jun 2;10:43. doi: 10.1186/1471-2121-10-43

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Copyright © 2009 Park et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Overexpression of a Bub2 construct slows growth. A. Cells grown on YPD at 30°C for two days. B. Cells grown on YPGR at 30°C for two days. 1.) wild type (KSY3), 2.) wild type with GAL1-BUB2 (KSY129), 3.) bub2Δ with GAL1-BUB2 (KSY130), 4.) bfa1Δ with GAL1-BUB2 (KSY169) 5.) wild type with GAL1-Tem1 and GAL1-Bub2 (KSY171). C. GAL1-T7-Bub2-6His is expressed in galactose and repressed in glucose. Anti-T7 blot of protein extracts lane 1: wild type with GAL1-BUB2 (KSY129) grown in YPD, lane 2: wild type with GAL1-BUB2 (KSY129) grown in YPGR, lane 3: bub2Δ with GAL1-BUB2 (KSY130) grown in YPD, lane 4: bub2Δ with GAL1-BUB2 (KSY130) grown in YPGR, lane 5: bfa1Δ with GAL1-BUB2 (KSY169) grown in YPD, lane 6: bfa1Δ with GAL1-BUB2 (KSY169) grown in YPGR. D. Expression of GAL1-TEM induced by galactose and repressed by glucose. Anti-myc blot of protein extracts lane 1: GAL1-Tem1 GAL1-Bub2 (KSY171) in YPD, lane 2: GAL1-Tem1 GAL1-Bub2 (KSY171) in YPGR.