Figure 3.

TGF-β₁ and CO increase Sprr1a expression in vitro and in vivo. (A) Western blot of mouse lung fibroblasts stimulated with TGF-β₁ (2 ng/ml) and exposed to CO or control incubator air. (B) Sprr1a mRNA expression increases in whole-lung homogenate in response to bleomycin and CO (7 d). (C) Sprr1a protein expression is increased in whole-lung homogenate 7 and 14 days after bleomycin exposure. (D) Sprr1a protein expression is further increased in whole-lung homogenate of lungs of mice exposed to CO.