Table 2.
Virus detection and molecular quantitation of H9N2 AIV in the organs of experimentally infected broiler chicks at different days post inoculation plus mortality & HI antibody titre
| Days post inoculation | Samples | Mortality | HI Test (log 2) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Trachea | Lungs | Kidneys | Spleen | Faeces | Pancreas | Blood | |||
| 1 | 0/5a | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/40b | 2 |
| 2* | 0/1 | 0/1 | 1/1 (657) | 0/1 | 0/1 | 0/1 | - | 1/35 | - |
| 3 | 2/5 (22660)c | 1/5 (3.79×104) | 2/5 (439) | 1/5 (710) | 0/5 | 0/5 | 0/5 | 0/34 | 2 |
| 6 | 1/5 (l.43×l05) | 3/5 (133805) | 3/5 (377.2) | 2/5 (6787.5) | 4/5 (2216840.75) | 0/5 | 0/5 | 0/29 | 4 |
| 6* | 1/1 (l.07×l07) | 1/1 (l.52×l06) | 0/1 | 1/1 (18816) | 1/1 (6.66×108) | 0/1 | - | 1/29 | - |
| 9 | 0/5 | 0/5 | 5/5 (735.2) | 0/5 | 0/5 | 0/5 | 0/5 | 0/23 | 8 |
E =Experimental group
*
= Dead bird
a
No. of samples positive for virus recovery/total samples
b
No. of chicken/total
c
Mean of positive samples