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. 2009 Jul 3;4(7):e6125. doi: 10.1371/journal.pone.0006125

Figure 1. Efficient inhibition of EGFP expression by zebrafish mir-30e precursor in vivo.

Figure 1

(A, B) Diagram of zebrafish miR-30e precursor sequence (A), and the EGFP sensor EGFP-2×PTmir30e containing two tandem perfectly complementary target sites for miR-30e binding (2×PT) in its 3′UTR (B, upper). The capped EGFP sensor mRNAs were co-injected with either miR-30e or miR-155 precursor mRNAs into one-cell stage embryos (B, bottom). (C) A dramatic decrease of EGFP fluorescence in 24 hpf embryos co-injected with EGFP-2×PTmir30e sensor (10 pg) and miR-30e precursor (50 pg). The DsRed mRNA was co-injected as an injection control (bottom panels). (D) Western blot analysis of 24 hpf embryos injected with EGFP-2×PTmir30e sensor and miR-155 precursor or miR-30e precursor. The upper non-specific bands were shown as a loading control.