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. 2009 Jul 3;4(7):e6125. doi: 10.1371/journal.pone.0006125

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Dong et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The expression of DsRed fluorescence and transcripts recapitulates the endogenous lmo2 expression pattern in the Tg(zlmo2:mir-shRNA^gata-1-actin-DsRed-BGH) ^{line 3}. Note that stronger expression of DsRed transcripts can be observed in the PBI region than in the ICM region. ICM: intermediate cell mass; PBI: posterior blood island. (B) WISH analysis of gata-1 expression in the DsRed⁻ and DsRed⁺ F1 siblings at 22 hpf. Red star denotes the gata-1 staining in the developing kidney. (C) WISH analysis of pu.1 expression in the DsRed⁻ and DsRed⁺ F1 siblings at 22 hpf. Note that the pu.1 staining is massively increased (arrow). All embryos are dorsal view with head to the left.