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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Dev Biol. 2009 Apr 1;330(1):167–174. doi: 10.1016/j.ydbio.2009.03.022

Fig. 1.

Fig. 1

Inactivation of Nxf2 causes male meiotic arrest in mice of mixed (C57BL/6 × 129) backgrounds. (a) The Nxf2 targeting construct and various Nxf2 alleles. The mouse Nxf2 gene consists of 23 exons and spans a 22-kb genomic region on the X chromosome. In the Nxf2flox allele, one loxP site is inserted in intron 2 and one in intron 11. Exons 3-11 encode amino acids 44-345. (b) Western blot analysis of adult wild type and Nxf2-/Y testes. Equal amounts (30 μg) of testis protein extracts were loaded. Three blots were probed with anti-NXF2, anti-NXF1, and anti-β-actin antibodies respectively. The abundance of NXF1 did not differ between Nxf2-/Y and wild type testes. (c) Testis weight of adult Nxf2-/Y mice. Adult Nxf2-/Y males have either small or large testes. The average testis weight ± standard deviation is shown for each group (22 mice each). (d, f) Histological analysis of testes from adult wild type mice. (e, g) Histological analysis of small testes from 4-month-old Nxf2-/Y mice reveals meiotic arrest. Arrows indicate normal chromosome segregation in wild type anaphase I spermatocytes (f) and chaotic chromosome segregation in Nxf2-/Y anaphase I spermatocytes (g). Scale bars, 50 μm.