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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Dev Biol. 2009 Apr 23;331(1):26–37. doi: 10.1016/j.ydbio.2009.04.018

Figure 2.

Figure 2

Activation of the Hh pathway. The Hh pathway was activated by three treatments, injection of a Ptc MASO which blocked Ptc activity thereby allowing Smo to be active (B, E), injection of RNA construct that produced constitutively activated Smo (C,F,H), or over-expression of Hh (I). At 18 hr. the Ptc MASO (0.75 mM) delayed gastrulation (B) relative to controls (A), but development caught up and at 48 hr the phenotype was mild with an increased number of pigment cells (E), relative to controls (D). Activated Smo did not delay gastrulation at 0.63 pg/pl with ca. 1 pl injected per egg. However, at 48 hr there were an excess number of pigment cells (F), an abnormal skeletal pattern and abnormal circumesophageal musculature (H), relative to controls at 48 hr (D, G). At 48 hr, embryos expressing extra Hh (ca.1 pl at 0.58 pg/pl) displayed an abnormal skeleton (I).