Skip to main content
. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Dev Biol. 2009 Apr 23;331(1):26–37. doi: 10.1016/j.ydbio.2009.04.018

Figure 4.

Figure 4

In situ analysis of non-skeletogenic mesoderm markers at the mesenchyme blastula stage. For each in situ imaged both a lateral view (A–C, G–I, M–O), and a vegetal view (D–F, J–L, P–R) are shown. (A,D,G,J,M,P) control embryos; (B,E,H,K,N,Q) embryos injected with Ptc MASO (0.75 mM); (C,F,I,L,O,R) embryos injected with Activated Smo (0.63 pg/pl). AA29 is a marker for all non-skeletogenic cells; GCM is an early activator of NSM and necessary for pigment cell specification; Scl, is a Marker activated prior to mesenchyme stage in NSM and necessary for blastocoelar cell specification. Both the Ptc MASO and Activated Smo had phenotypes at the pluteus stage but had no noticeable effect on expression of these three NSM markers at the mesenchyme blastula stage.