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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Dev Biol. 2009 Apr 23;331(1):26–37. doi: 10.1016/j.ydbio.2009.04.018

Figure 5.

Figure 5

Hedgehog knockdowns do not affect expression of endomesoderm GRN components at late gastrula stage. GCM normally is expressed in the aboral NSM and in pigment cells at late gastrula (A). Embryos injected with Hh MASO at concentrations that affect late patterning nevertheless express GCM normally (B,C). PKS is a differentiation gene in the pigment cell lineage and pigment cells invade ectoderm on the aboral side (D). Hh MASO knockdowns do not alter PKS expression nor the ectodermal invasion (E,F). AA29 stains a number of NSM cells at late gastrula (G), and that staining pattern is not significantly altered in Hh MASO knockdowns (H,I). vv = ventral view.