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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Mech Dev. 2009 Mar 4;126(5-6):337–349. doi: 10.1016/j.mod.2009.02.005

Figure 4. Phosphorylated p38MAPK is observed in cells of the ureteric bud when NRAGE is overexpressed using Hoxb7-NRAGECherry transgenic mice.

Figure 4

Transgenic mice were generated to overexpress NRAGE in cells of the ureteric bud using the Hoxb7 promoter fragment and tagged with the mCherry fluorescent protein (Hobx7-NRAGECherry). Presented are representative images from the immunofluorescent staining of kidneys from E17 transgenic mice. (A-B) Immunofluorescent staining of E17 Kidneys with Dolichos Bifluorous (Green) and TOPRO-3 (Blue). (A) Hoxb7-NRAGECherry E17 kidneys stained with Dolichos Bifluorous Agglutinin (Green; ureteric bud cells), and TOPRO-3 (Blue; nuclei). Note the overlay of mCherry fluorescent protein (Red) with Dolichos Bifluorous Agglutinin (Green) as Yellow, demonstrating that mCherry is indeed expressed in cells of the ureteric bud. (B) Wild-type E17 kidneys stained with Dolichos Bifluorous Agglutinin (Green; ureteric bud cells) and TOPRO-3 (Blue; nuclei). Note that no mCherry protein (Red) is expressed in cells of the ureteric bud (Green). (D,F,H,J,L) Wild-Type E17 kidneys: (D) stained with antibody to p38MAPK (p38MAPK; Green), (F) stained with antibody to phosphorylated p38MAPK (p38MAPK-P; Green), (H) expression of mCherry fluorescent protein (Red), (J) stained with DAPI (nuclei; Blue), (L) overlay of panels F (phosphorylated p38MAPK), H (mCherry), and J (DAPI). Note panels F, H, J, and L are all images taken from the same section of a wild-type E17 kidney. (C,E,G,I,K) Hoxb7-NRAGECherry E17 kidneys: (C) stained with antibody to p38MAPK (E) stained with antibody to phosphorylated p38MAPK (p38MAPK-P), (G) expression of mCherry fluorescent protein, (I) stained with DAPI, (K) overlay of panels E (phosphorylated p38MAPK), G (mCherry), and I (DAPI). Note panels E, G, I, and K are all images taken from the same section of a transgenic Hoxb7-NRAGECherry E17 kidney. Note: (Panels A and B): ureteric bud cells (Green; DBA), mCherry fluorescent protein (Red), Nuclei (Blue; TOPRO-3). (Panels C through L): p38MAPK (Green; Alexa-546), phsophorylated p38MAPK (Green; Alexa-546), mCherry fluorescent protein (Red), Nuclei (Blue; DAPI), Overlay of phosphorylated p38MAPK and mCherry (Red) as Yellow. Scale bar = 50 microns.