Figure 5. Myo4-immunization induces strong delayed-type hypersensitivity responses specific for Myo4, purified cardiac myosin, and Allen peptide.
Cellular immune responses to Myo4, myosin, and Allen peptide were measured by a standard 24 hr ear swelling, delayed-type hypersensitivity (DTH) assay, in mice immunized with myosin, Myo4, Allen peptide or PBS. Right ears were injected with 10 μg of test antigen and left ears were injected with 10 μg BSA and net ear swelling (right minus left) was determined. Error bars, when shown, represent SEM. The absence of an error bar indicates that the SEM could not be calculated due to an insufficient number of measurements. * p ≤ 0.05.