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. Author manuscript; available in PMC: 2009 Sep 1.

Published in final edited form as: Autoimmunity. 2008 Sep;41(6):490–499. doi: 10.1080/08916930802167902

(A) Myo4-specific production of IFN-γ (Th1), IL-4 (Th2), IL-6 and IL-17 (Th17) was analyzed using a standard ELISPOT assay. Following the isolation of splenocytes from Myo4-immunized animals at days 7, 10, 14, 17 and 21 days post immunization, cells were stimulated with purified Myo4 (10 μM) for 24 hrs for IFN-γ, IL-6, and IL-17 or 48 hrs for IL-4. (B) For a more detailed investigation of cytokine production, intracellular staining with antibodies specific for IFN-γ, IL-4 and IL-17 was performed. CD90.2+ T cells were analyzed from splenocyte (SP) and lymphocyte (LN) preparations from either Myo4- or PBS-immunized mice. All experiments were repeated at least three times. For ELISPOT analysis, error bars represent SEM.

(A) Myo4-specific production of IFN-γ (Th1), IL-4 (Th2), IL-6 and IL-17 (Th17) was analyzed using a standard ELISPOT assay. Following the isolation of splenocytes from Myo4-immunized animals at days 7, 10, 14, 17 and 21 days post immunization, cells were stimulated with purified Myo4 (10 μM) for 24 hrs for IFN-γ, IL-6, and IL-17 or 48 hrs for IL-4. (B) For a more detailed investigation of cytokine production, intracellular staining with antibodies specific for IFN-γ, IL-4 and IL-17 was performed. CD90.2+ T cells were analyzed from splenocyte (SP) and lymphocyte (LN) preparations from either Myo4- or PBS-immunized mice. All experiments were repeated at least three times. For ELISPOT analysis, error bars represent SEM.