Table 5. Monoclonal antibodies (mAbs) used in surveys 1 and 2. Source: BC, Beckman Coulter (Fullerton, CA, USA); BD Becton Dickinson (Franklin Lakes, NJ, USA).
Panels survey 1 | Antibodies | Fluorescence | Sources |
CD19/CD40/CD3 | J4.119/MAB89/UCHT1 | FITC/PE/PC5 | BD/BC/BC |
CD19/CD56/CD3 | J4.119/N90/UCHT1 | FITC/PE/PC5 | BD/BC/BC |
CD4/CD8 | 13B8.2/B9.11 | FITC/PC5 | BC |
CD4/CD25/CD8 | 13B8.2/B1.49.9/B9.11 | FITC/PE/PC5 | BC |
CD3/CD25/CD8 | UCHT1/B1.49.9/B9.11 | FITC/PE/PC5 | BC |
Panels survey 2 | Antibodies | Fluorescence | Sources |
CD4/CD8 | 13B8.2/B9.11 | FITC/PC5 | BC |
CD3/CD8/CD45RA/CD62L | UCHT1/SK1/5H9/SK11 | APC/PerCP/FITC/PE | BD |
CD3/CD4/CD45RA/CD62L | UCHT1/L200/5H9/SK11 | APC/PerCP/FITCPE | BD |
CD3/CD8/CD45RA/CD28 | UCHT1/SK1/5H9/CD28.2 | APC/PerCP/FITC/PE | BD |
CD3/CD4/CD28/HLA DR | UCHT1/L200/SK1/G46-6 | APC/PerCP/FITC/PE | BD |
CD3/CD4/CD28/CD95 | UCHT1/L200/SK1/UB2 | APC/PerCP/FITC/PE | BD |
CD8/CD69/CD16 | RPA-T8/L78/3G8 | APC/PerCP/PE | BD/BD/BC |
CD19/CD69/CD95 | H1B19/L78/UB2 | APC/PerCP/PE | BD |
Fluorescein-isothiocyanate (FITC), phycoerythrin (PE), phycocyanin (PC5) peridinin chlorophyll rotein (PerCP), or allophycocyanin (APC). Negative controls were performed with unrelated murine mAbs (BC and BD).