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. 2009 Jul 10;5(7):e1000513. doi: 10.1371/journal.ppat.1000513

Figure 2. Origin interaction domain (OID) is required but not sufficient for ssDNA-dependent Rep68.

Figure 2

Rep40 and OID proteins (16.6 µM) were incubated in the absence (A and D) or presence of 2.8 (B and E) or 16.6 µM ssDNA (C and F). OID was also incubated in the presence of 2.8 (G) or 16.6 µM RBS dsDNA (H). Fifty µL of sample was chromatographed on a Superdex 200 10/300 GL column with a flow rate of 0.5 mL/min. Protein elution was followed by UV detection at 280 nm. Molecular weight standards are shown on top, and dashed line corresponds to the elution position of Rep protein alone.