Table 4.
Western blot densitometry of Akt and phosphor-Akt (Ser473) and AMPK and phosphor-AMPK (Thr172) in the heart (measured in arbitrary units). Data are presented as the fold change in protein expression relative to the STD Sham group.
STD | HFD | |||
---|---|---|---|---|
Protein | Sham n = 7 | TAC n = 8 | Sham n = 10 | TAC n = 9 |
Phospho-Akt | 1.00 ± 0.08 | 0.83 ± 0.06 | 0.90 ± 0.05 | 0.83 ± 0.02 |
Total Akt | 1.00 ± 0.06 | 0.99 ± 0.06 | 0.94 ± 0.05 | 1.02 ± 0.02 |
Phospho-Akt/Total Akt | 1.00 ± 0.07 | 0.86 ± 0.08 | 0.98 ± 0.09 | 0.84 ± 0.02 |
Phospho-AMPK | 1.00 ± 0.07 | 0.87 ± 0.06 | 0.92 ± 0.06 | 0.87 ± 0.02 |
Total AMPK | 1.00 ± 0.06 | 0.99 ± 0.09 | 1.02 ± 0.03 | 1.25 ± 0.03 |
Phospho-AMPK/Total AMPK | 1.00 ± 0.08 | 0.89 ± 0.06 | 0.88 ± 0.07 | 0.73 ± 0.02 |