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. Author manuscript; available in PMC: 2009 Jun 26.
Published in final edited form as: J Neurochem. 2005 May;93(3):698–705. doi: 10.1111/j.1471-4159.2005.03091.x

Fig. 2.

Fig. 2

Effect of perinatal Cu deficiency on brain iron concentration in P24–P26 male and female rats. Iron was determined by flame atomic absorption spectroscopy in non-perfused and perfused rats. Each bar represents the mean ± SEM (n = 4). Open bars represent values for Cu-adequate rats and shaded bars for Cu-deficient rats. Perfusion lowered the brain iron concentration in both Cu-adequate and Cu-deficient rats of both genders, p < 0.05. Brain iron was determined in another set (n = 4) of non-perfused littermates but was corrected for blood Fe contamination after measurement of hemoglobin and estimation of blood iron. These values were not different from those measured in perfused littermates.