Table 1.

Changes in Host Immune Response and Defense Genes Upregulated in the HSV-1 17Syn⁺ Latent TG Relative to 17ΔPst(LAT⁻) Latent TG 1 Hour after Mouse Hyperthermic Stress

		Change (x-Fold)
Affymetrix Probe Number*	Gene Symbol (Name)	Microarray	Real-Time PCR
1427837_at	IgK-V32 (immunoglobulin kappa chain variable 32 (V32))	+7.9	+1.51
1424931_s_at	IgL-VI (immunoglobulin lambda chain, variable 1)	+3.6	+13.59
1430523_s_at	IgL-VI homologue (immunoglobulin lambda chain, variable 1)	+3.4	+2.17
1425324_x_at	IgH-4 (serum IgG1) (immunoglobulin heavy chain 4)	+2.9	+8.40
1416111_at	CD83 (CD83 antigen)	+3.3	+17.39
1444078_at	CD8A (CD8 antigen, alpha chain)	+2.9	+2.95
1419684_at	CCL8 [chemokine (C-C motif) ligand 8]	+3.0	+3.05
1417976_at	ADA (adenosine deaminase)†	+5.3	+9.65
1428719_at	2010309G21RIKIgL (RIKEN cDNA 2010309G21 gene immunoglobulin lambda chain complex)	+3.0	+1.04

Changes in expression of nine genes related to host immune response and defense based on microarrays and quantitative real-time PCR in the 17Syn⁺ high phenotypic reactivator latent TG relative to a LAT⁻ recombinant, 17ΔPst low phenotypic reactivator latent TG 1 hour after mouse hyperthermic stress. Relative quantitative expression levels were determined for each gene. All results are expressed as an expression ratio of the 17Syn⁺ latent TG to the 17ΔPst(LAT⁻) latent TG of heat-treated mice, normalized against β-actin expression levels using the 2^−ΔΔC_T method.⁴³

^*Affymetrix, Inc., Santa Clara, CA.

^†Pathway for Ada upregulated in the purine nucleotide metabolism.