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. Author manuscript; available in PMC: 2009 Jun 26.
Published in final edited form as: Cell Signal. 2007 Jan 10;19(6):1185–1192. doi: 10.1016/j.cellsig.2006.12.010

Fig. 2. Co-immunoprecipitation of HA-GPS1 with Myc-Asb-4 in HEK293 cells.

Fig. 2

A. pCMV-HA-GPS1 (full length) vector was co-transfected with either pCMV empty vector or pCMV-Myc-Asb-4 vector in HEK293 cells. Cell lysates were immunoprecipitated with anti-Myc antibody-conjugated agarose and blotted with either anti Asb-4 antibody or anti HA antibody. B. pCMV-Myc-Asb-4 vector was co-transfected with either pCMV empty vector or pCMV-HA-GPS1 (full length) vector in HEK293 cells. Cell lysates were immunoprecipitated with anti-HA antibody-conjugated agarose and blotted with either anti GPS1 antibody or anti-Myc antibody.