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. Author manuscript; available in PMC: 2009 Jun 26.
Published in final edited form as: Cell Signal. 2007 Jan 10;19(6):1185–1192. doi: 10.1016/j.cellsig.2006.12.010

Fig 4. Effect of Asb-4 on the protein level of GPS1 in HEK293 cells.

Fig 4

A. HEK293 cells were co-transfected with 1 μg (35 mm dish) of pCMV-HA-GPS1 vector along with differing amounts of pCMV-Myc-Asb-4 vector. Cell lysates were blotted with anti-HA antibody. B. The HEK293 cells were co-transfected with 1 μg pEGFP vector along with differing amounts of pCMV-Myc-Asb-4 vector. Cell lysates were blotted with anti EGFP antibody. C. HEK293 cells were co-transfected with 1 μg of pCMV-HA-GPS1 vector along with differing amounts of pCMV-Myc-Asb-4/Δsb vector. Cell lysates were blotted with anti-HA antibody. The total amount of vector DNA in each transfection was equalized with pCMV empty vector. The membranes were stripped and reblotted with anti-Myc and anti-β-actin antibodies respectively. *p < 0.05, **p < 0.01 vs. control.