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. Author manuscript; available in PMC: 2009 Jun 26.
Published in final edited form as: Cell Signal. 2007 Jan 10;19(6):1185–1192. doi: 10.1016/j.cellsig.2006.12.010

Fig. 5. GPS1 was not ubiquitinized by Asb-4.

Fig. 5

Lane 1: HEK293 cells were co-transfected with Myc-ub and HA-IRS1. Lane 2: HEK293 cells were co-transfected with Myc-ub, HA-IRS1 and SOCS1. Lane 3-5:HEK293 cells were co-transfected with Myc-ub, HA-GPS1, Myc-Asb-4 or Asb-4/Δsb as indicated. Total amount of DNA in each transfection was equalized with empty vector. Cell lysates were mmunoprecipitated with anti-HA antibody and blotted with anti-Myc antibody. Membranes were stripped and reblotted with anti-HA antibody.