. Author manuscript; available in PMC: 2010 May 1.

Published in final edited form as: J Med Entomol. 2009 May;46(3):496–504. doi: 10.1603/033.046.0313

Table 1.

Oligonucelotide primers used to amplify and clone AaHsp70 paralogs

Primers^a	Sequence (5′ to 3′)	Expected length (bp)
Aa_F	ATCAAGATTGTCGACCAACTAGAAGGACC	2,398
Aa_R	TTTCTACTATATAAGCGCCCGGTTCG
Ab_F	TCGTTTCCACTATATAAGCGCCCAGC	2,346
Ab_R	CTGAGTTCGGTATGTTTGAGAATGAGAATG
Ba_F	CGCGTCTTCCAACATTCCTTACTGAACCTA	2,374
Ba_R	CCACTCACACAAAGCAATGAAATATGCGAC
Bb_F	CGAATCACAAGCAAGAGCAATAAAGCGCC	2,463
Bb_R	GGCAATTTCGTAGCTCTATTGACATGTCCAG
Ca_F	GGTAGCAGTCGAGTAAGCCAAGACAACGAA	2,334
Ca_R	AACAAGCTCAACATAATAGGAACATTAACAAACTTCC
Cb_F	CCTGGCGTTTATATATAGGACCGATTCGAGC	2,374
Cb_R	AAGCATTAGCTTGAGTCGTCAATCTTTAAGATTACTG

Cloning primers contained the sequence ttttggtacc at the 5′ end of each of the above oligonucleotides to introduce KpnI restriction sites.