Table 1.
Composition of the human microbiota compared in three age groups
| TaqMan detection | SYBR-Green detection | |||||||
|---|---|---|---|---|---|---|---|---|
| Firmicutes | Firmicutes | Firmicutes | ||||||
| n | All-bacteria (a) | C. leptum group (b) | C. coccoides group (b) | Bacteroides/Prevotella group (b) | Bifidobacterium genus (b) | E. coli (b) | Lactobacillus/Leuconostoc/Pediococcus group (b) | |
| Infant | 21 | 10.7 ± 0.1 (A) | -3.2 ± 0.4 (A) | -3.2 ± 0.4 (A) | -1.5 ± 0.3 (A) | -0.6 ± 0.2 (A) | -1.5 ± 0.3 (A) | -3 ± 0.2 (A) |
| Adult | 21 | 11.5 ± 0.1 (B) | -0.7 ± 0.1 (B) | -1.2 ± 0.1 (B) | -1.5 ± 0.1 (AB) | -2.3 ± 0.3 (B) | -3.8 ± 0.1 (B) | -3.9 ± 0.3 (AB) |
| Elder | 20 | 11.4 ± 0.1 (B) | -1.1 ± 0.1 (C) | -1.8 ± 0.1 (A) | -1 ± 0.1 (A) | -2.3 ± 0.3 (B) | -2.4 ± 0.2 (C) | -4.2 ± 0.2 (B) |
n represents the number of samples in each group.
(a) All-bacteria results obtained by qPCR were expressed as the mean of the log10 value ± SEM.
(b) Results were expressed as the mean of the log10 value ± SEM of normalized data calculated as the log of targeted bacteria minus the log of All-bacteria number.
The non parametric Wilcoxon test was performed.
Data not sharing the same letter within a column are significantly diferrent at p < 0.05.