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. 2009 Jun 11;10:18. doi: 10.1186/1471-2091-10-18

Table 2.

Single stranded DNA substrates used

Substrate Source Primers Substrate size (bases) %GC
PBAD pBAD/thio Left-ATGCCATAGCATTTTTATCG
Right-*GACCGGTACGCGTAGAATCG		 556 47
MdnaK M. tuberculosis dnaK Left-*GAACCCGTTGTTCTTAGACGAG
Right-*GGGTAACATCAAGCAGCAGAAC		 317 62
Mtop M. tuberculosis topA Left-*GTAGAAGTTGTTGAGCCAGT
Right-TACTCGTCGATCATCAAGAC		 216 61

* Primer 32P labeled at 5'end to analyze extended strand as cleavage substrate after denaturation of the PCR product. The left and right primers of MdnaK were utilized in separate experiments.