Figure 1.

HT-RNAi kinase screening for identification of sensitizers to gemcitabine. HT-RNAi screens were performed on MIA PaCa-2 cells transfected with a siRNA library targeting 572 kinases followed by treatment with either vehicle or 5 nM or 10 nM gemcitabine. Cell viability was assessed and normalized to control wells. (A) Scatterplot of the log₂ values of cell viability for gemcitabine plus siRNA treated cells versus vehicle plus siRNA treated cells showed CHK1 as a significant hit. (B) Plot of log₂ ratios of gemcitabine/vehicle for each siRNA treated with either 5 nM or 10 nM gemcitabine. (C) Empirical Probability Distribution of log₂ ratios of gemcitabine/vehicle (5 nM and 10 nM). Hit areas are highlighted in red. (D) Venn diagram of gene hits from both the 5 nM (highlighted in pink) and 10 nM (highlighted in yellow) gemcitabine synthetic lethal RNAi screen.