Figure 4.

Expression study by Real-time RT-PCR of a few unigenes varying in EST redundancy. Real-time PCR (qRT-PCR) was conducted for five unigenes encoding JAIP, catalase, PEAMT, P5CS and DnaJ to see the effect of NaCl treatment on their expression. qRT-PCR was also performed for BADH. Actin gene served as the internal control. RNA isolated from the leaves of control and NaCl treated plants was individually set for qRT-PCR using QuantiFast SYBR Green RT-PCR kit (Qiagen, USA) and 1 μM gene-specific primer. Each bar represents the number of fold increase in the transcript level of a gene in the plant upon NaCl treatment compared to the control level. The values presented are the mean ± standard deviation (sd) of three independent experimental analysis.