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. Author manuscript; available in PMC: 2009 Aug 8.
Published in final edited form as: Mol Cell. 2008 Aug 8;31(3):383–394. doi: 10.1016/j.molcel.2008.06.018

Figure 4. Activation of PLC-β2 by G Protein Activators Is Retained in PLC-β2 Lacking the Majority of the X/Y Linker.

Figure 4

COS-7 cells were transfected with DNA encoding either PLC-β2 or the indicated PLC-β2 deletion forms in the presence or absence of 30 ng of constitutively active Rac3(G12V) (A), 150 ng of each subunit of Gβ1γ2 (B), or 100 ng of Gαq (C) prior to quantification of [3H]inositol phosphates and vector subtraction. All transfections used 30 ng of each PLC form except for (C), where 10 ng of each PLC was transfected. Endogenous activity resulting from transfection of either G protein or Rac3(G12V) alone is indicated by the first bar on each plot. Data are the mean ± SD of triplicate samples and are representative of results from three or more experiments. PLC-β2 expression was assessed by western blotting (insets).