Table 3.
Relative levels of histone proteins in MCF-7 cells after treatment with estrogenic compounds.
| Relative histone level (mean ± SE, n = 4)
|
|||||||
|---|---|---|---|---|---|---|---|
| H2A
|
H2B
|
H3
|
H4
|
||||
| Treatment | SELDI | SELDI | IB | SELDI | IB | SELDI | IB |
| E2 | 2.3 ± 0.6 | 2.1 ± 0.3 | 2.3 ± 0.1 | 3.1 ± 0.3 | 2.9 ± 0.2 | 2.1 ± 0.4 | 2.6 ± 0.1 |
| GEN | 2.2 ± 0.3 | 2.2 ± 0.6 | 2.2 ± 0.2 | 2.5 ± 0.4 | 2.6 ± 0.3 | 2.1 ± 0.4 | 2.8 ± 0.3 |
| BPA | 2.1 ± 0.5 | 2.1 ± 0.1 | 2.2 ± 0.1 | 2.6 ± 0.3 | 2.2 ± 0.2 | 2.1 ± 0.2 | 2.7 ± 0.4 |
| EDS | 2.3 ± 0.2 | 2.0 ± 0.5 | 2.4 ± 0.1 | 2.4 ± 0.2 | 2.8 ± 0.2 | 2.2 ± 0.6 | 2.6 ± 0.2 |
We treated cells with 20 pM E2, 1 μM GEN, 1 μM BPA, or 10 μM EDS for 6 days, and compared the levels of histones with those of vehicle-treated cells by SELDI-TOF MS (SELDI) and immunoblotting (IB). Immunoblotting data was not obtained for histone H2A, as no suitable antibody was available. Both methods performed similarly; there were no statistically significant differences between levels of histones determined by SELDI-TOF MS or immunoblotting. p > 0.05 in all cases by Student’s t-test.