Fig. 11.

Western blotting of extracted induced threads using polyclonal anti-recombinant thread matrix proteins (rTMP) antibodies. Acetic acid extracts of induced threads (∼10 μg) were separated on an acid–urea polyacrylamide gel electrophoresis (AU PAGE) gel (A) or an sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) gel (B) and processed for western blotting as described in the Materials and methods. Primarily, one band stains in AU (acid–urea) whereas there are two on SDS PAGE, of which the slower seems likely to be a dimer. The arrows in A correspond to the proteins that were partially purified in Fig. 2. CBBR, Coomassie Brilliant Blue R.