Table 1.
Composition of distal threads, extracts and partially purified thread matrix proteins (TMPs)
| Amino acid | Distal threada | Acid extracts | Purified proteins | TMPs | TMPs calc. |
|---|---|---|---|---|---|
| Hyp | 5.80 | ND | ND | ND | 0 |
| Asx | 5.17 | 11.50 | 12.01 | 17.27 | 20.6 |
| Thr | 3.09 | 3.62 | 3.09 | 2.82 | 2.7 |
| Ser | 4.75 | 6.02 | 4.17 | 3.92 | 2.3 |
| Glx | 5.21 | 6.64 | 5.45 | 2.80 | 0 |
| Pro | 7.74 | 5.86 | 4.82 | 1.67 | 0 |
| Gly | 34.17 | 27.97 | 32.69 | 33.01 | 31.2 |
| Ala | 16.21 | 8.58 | 6.51 | 2.92 | 0 |
| Cys/2 | 0.13 | ND | ND | ND | 0 |
| Val | 2.44 | 2.06 | 2.38 | 2.73 | 3.1 |
| Met | 0.63 | ND | 0.48 | 0.22 | 0 |
| Ile | 1.01 | 1.06 | 2.09 | 3.47 | 6.4 |
| Leu | 2.91 | 1.77 | 2.05 | 1.39 | 0.6 |
| DOPA | 0.18 | 3.26 | 2.97 | 2.51 | – |
| Tyrb | 1.50 (1.68) | 5.42 (8.68) | 10.05 (13.02) | 15.15 (17.66) | 23.4 |
| Phe | 1.09 | 0.97 | 1.38 | 1.09 | 0.8 |
| His | 2.19 | 2.35 | 1.10 | .92 | 0.8 |
| Hyl | 0.01 | ND | ND | ND | 0 |
| Lys | 3.76 | 4.88 | 4.51 | 4.76 | 5.6 |
| Arg | 2.00 | 4.75 | 4.09 | 3.35 | 2.5 |
Values represent mean residues per 100 residues from at least two samples. aLucas et al., 2002. bNumber in parentheses is Dopa residues + tyrosine (Tyr) residue for the total Tyr amount. Acid extracts are acetic acid soluble proteins from distal byssal threads. Purified protein refers to the RP-HPLC purified group of proteins. `TMPs' are the most abundant group of proteins found in the partially purified TMPs. `TMP's Calc.' is the calculated amino acid composition of the assembled full-length TMP-1 variant. The detection of Dopa was variable due to experimental procedure. In order to achieve accurate total Tyr compositions, only compositions in which Dopa was detected were included