Table 3.
Quantification of IsoAspartate (IsoAsp), deamidation and the IsoAsp:Aspartate (IsoAspartate:aspartate) ratio in aged recombinant thread matrix proteins (rTMP)
| rTMP treatment | IsoAsp (mol/mol ±s.d.) | Deamidation (mean/peptide) | IsoAsp:Asp (approximate) |
|---|---|---|---|
| Non-aged | Da,b | 1.4 (4.2)c | – |
| 37°C 3 days | 1.22±0.10b | 4.9 (14.7)c | 1:3 |
| 37°C 6 days | 1.81±0.09b | 5.7 (17.1)c | 1:2 |
| 14°C 6 days | 0.19±0.03b | 2.4 (7.2)c | 1:12 |
a
None detected
b
N=3
c
Number in parentheses is the estimated number of deamidation events for a full-length TMP