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. 2009 Jun 26;212(14):2261–2268. doi: 10.1242/jeb.025718

Fig. 3.

Fig. 3.

Ultrastructure of Leptopilina spp. venom gland. (A,B) Confocal images of L. heterotoma venom gland, in which the rough (R) and the smooth (S) parts of the canals are distinguishable by the different TRITC-phalloidin staining intensity. L, gland lumen. Objective: Plan-Apochromat ×63/1.4, oil DIC optics. (C) Transmission electron micrograph of one L. victoriae F-actin-lined canal, with rough, microvillar (R) and smooth, cuticle-lined (S) portions. Cross-sectioned microvilli (mv) are visible in the rough portion and virus-like particle (VLP) precursors (white arrows) are found in the canal lumen. Scale bar, 1.1 μm. Magnification: ×7000, 80 KeV. (D) Transmission electron micrograph of a cross-section of one L. victoriae canal lined by microvilli (mv). The black arrow points to a putative mesh-like matrix; arrowheads indicate electron-dense structures between the microvilli. In addition, VLP precursors (white arrows) are observed in the canal lumen. Scale bar, 0.4 μm. Magnification: ×20,000, 80 KeV. (E) Transmission electron micrograph of one L. victoriae canal showing the region where the rough portion transitions into the cuticle-lined smooth portion (black arrowhead). The rough region is lined with a mesh-like matrix (arrow) abutting the microvilli (mv). Scale bar, 0.25 μm. Magnification: ×30,000, 80 KeV. (F) Transmission electron micrograph of one L. victoriae smooth canal meeting the gland lumen (L). VLP precursors (white arrows) and cuticle lining the canal (black arrowhead) are clearly visible. Scale bar, 0.25 μm. Magnification: ×30,000, 80 KeV.