Fig. 5.
GG 625/629 LL mutation does not impair APP-PS1 interaction. The interaction between APP, APP mut5 and PS1 was studied in CHO cells co-expressing APP and human PS1. A. The levels of APP (top), PS1 holoprotein and PS1 N-terminal fragment (NTF, bottom) were monitored by Western blotting revealed with the WO-2 and MAB1563 antibodies, respectively. B. Cell lysates were immunoprecipitated by the WO-2 (human APP) antibody and revealed by Western blotting with the human PS1-specific MAB1563 antibody. Arrows indicate the expected position of cellular PS1 holoprotein, and PS1 N-terminal fragment.