Table 1.
Summary of q PCR assay results from June 2007 samples.
| Station | D (m) | T (°C); S (‰) | Trichodesmium | Richelia–Rhizosolenia (het-1) | Richelia–Hemiaulus (het-2) | Calothrix–Chaetoceros (het-3) | Group A | Group B |
|---|---|---|---|---|---|---|---|---|
| 1 | 1.3 | 28.0; 33.4 | 2.1 × 104 (2.9 × 103) | nd (nd) | 1.86 × 102 (nd) | nd (nd) | nd (nd) | dnq (nd) |
| 40.4 | 22.5; 36.3 | nd (nd) | nd (nd) | nd (nd) | 5.5 × 102 (3.6 × 102) | nd (nd) | nd (nd) | |
| 2 | 1.2 | 25.6; 35.6 | 4.6 × 102 (1.7 × 105) | nd (nd) | 8.0 × 101 (8.4 × 102) | nd (nd) | 1.1 × 104 (1.3 × 103) | dnq (nd) |
| 38.2 | 22.0; 36.2 | 3.81 × 103 (dnq) | nd (nd) | 3.9 × 102 (nd) | 6.7 × 103 (nd) | 4.2 × 102 (nd) | nd (nd) | |
| 3 | 0 | 25.1; 36.2 | dnq (dnq) | 3.8 × 102 (nd) | 2.1 × 103 (4.0 × 103) | 1.6 × 103 (4.7 × 102) | 1.2 × 104 (4.1 × 103) | nd (nd) |
| 4 | 1.7 | 26.7; 33.8 | 8.0 × 103 (1.0 × 105) | dnq (nd) | nd (dnq) | 5.6 × 102 (nd) | dnq (nd) | nd (nd) |
| 29.3 | 26.4; 33.9 | 8.3 × 101 (nd) | nd (nd) | 2.3 × 102 (nd) | 1.0 × 102 (nd) | nd (nd) | nd (nd) | |
| 5 | 0 | 26.5; 33.1 | 2.5 × 104 (1.7 × 105) | 1.1 × 104 (6.3 × 102) | 3.3 × 103 (1.5 × 103) | nd (nd) | 7.0 × 102 (dnq) | nd (nd) |
| 6 | 0 | 26.5; 34.9 | 1.4 × 104 (4.1 × 103) | 1.3 × 103 (1.8 × 104) | 4.3 × 103 (1.8 × 104) | nd (nd) | nd (nd) | nd (b) |
| 39.3 | 19.4; 36.0 | dnq (nd) | nd (nd) | 2.3 × 102 (nd) | 4.6 × 103 (nd) | nd (nd) | nd (nd) | |
| 7 | 0 | 25.3; 35.8 | 3.7 × 102 (2.5 × 103) | 8.7 × 101 (1.4 × 103) | 5.0 × 101 (2.8 × 103) | nd (nd) | 9.5 × 103 (dnq) | dnq (nd) |
| 38.8 | 21.5; 35.8 | 3.7 × 101 (nd) | 4.2 × 101 (4.2 × 102) | 3.0 × 102 (nd) | 4.2 × 103 (nd) | 7.2 × 103 (dnq) | nd (b) | |
| 8 | 0 | 27.0; 34.7 | 1.7 × 104 (7.1 × 103) | nd (3.4 × 102) | 3.9 × 102 (4.8 × 103) | dnq (nd) | 2.7 × 104 (1.1 × 104) | 2.0 × 102 (a) |
| 16.5 | 26.9; 34.7 | 2.1 × 103 (1.1 × 103) | nd (8.8 × 102) | 5.3 × 102 (3.6 × 103) | dnq (nd) | 5.9 × 103 (dnq) | 3.8 × 101 (a) | |
| 36.0 | 19.6; 36.0 | 1.2 × 101 (nd) | nd (nd) | dnq (nd) | nd (nd) | nd (dnq) | dnq (b) | |
| 9 | 1.2 | 28.2; 34.6 | 4.6 × 104 (1.8 × 102) | nd (9.4 × 101) | 5.7 × 102 (3.1 × 103) | 1.3 × 103 (nd) | nd (dnq) | 2.0 × 103 (a) |
| 19.9 | 28.2; 34.6 | 2.1 × 103 (4.2 × 103) | nd (1.2 × 102) | 5.0 × 103 (8.1 × 103) | 1.4 × 103 (nd) | dnq (nd) | 5.2 × 103 (a) | |
| 39.3 | 25.4; 35.9 | 6.7 × 103 (2.5 × 103) | 3.9 × 101 (4.2 × 102) | 9.1 × 101 (6.6 × 103) | nd (nd) | 4.5 × 103 (2.0 × 103) | 3.3 × 102 (a) | |
| 10 | 1.1 | 27.8; 34.6 | 6.1 × 104 (5.7 × 103) | 1.7 × 103 (5.6 × 102) | 6.6 × 101 (9.6 × 103) | 1.4 × 103 (nd) | nd (nd) | 3.2 × 103 (a) |
| 18.3 | 27.8; 34.6 | 3.1 × 104 (1.2 × 103) | 6.0 × 102 (6.7 × 102) | 4.2 × 102 (7.7 × 102) | nd (nd) | dnq (nd) | 9.0 × 102 (a) |
a
Quantitative RT-PCR not processed since samples only collected in the daytime; see Results for details.
Mean nifH gene copy abundances are shown with transcript abundances for each target in parentheses. nd, not detected; dnq, detected not quantifiable; D, depth; T, temperature; S, salinity.