Table 2.
Comparison of methylglyoxal-stimulated d-lactate and l-lactate production by trypanosomatids. Parasites were incubated with or without methylglyoxal for 2 h prior to analysis. Data represent the mean ± SD of triplicate determinations. See Experimental procedures for further details.
| Lactate (μm) |
||||||
|---|---|---|---|---|---|---|
|
d-Lactate |
l-Lactate |
|||||
| Organism | Plus methylglyoxal | Minus methylglyoxal | Net | Plus methylglyoxal | Minus methylglyoxal | Net |
| L. major | 385 ± 9 | 49 ± 9 | 337 | 7 ± 0.6 | 12 ± 0.1 | −5 |
| T. cruzi | 303 ± 3 | 8 ± 0.8 | 295 | 9 ± 0.3 | 13 ± 0.1 | −4 |
| T. brucei procyclics | 18 ± 0.2 | 8 ± 0.1 | 11 | 141 ± 5 | 21 ± 0.1 | 120 |
| T. brucei bloodstream forms | 68 ± 9 | 50 ± 2 | 18 | 355 ± 42 | 134 ± 18 | 221 |