Table 3.
Comparison of GLO1 activity, methylglyoxal sensitivity and methylglyoxal metabolism in T. brucei wild-type and transgenic cell lines. ND, not determined.
| Cell line | GLO1 activity (nmol·min−1· mg−1) | EC50a (μm) | Methylglyoxal metabolizedb (nmol·mL−1·h−1) |
|---|---|---|---|
| T. brucei | |||
| Procyclics | < 5 | 53.4 ± 2.9 | 246 ± 21 |
| Bloodstream forms | < 5 | ND | 300 ± 32 |
| pLew100–luciferasec | |||
| Procyclics | < 5 | 47.8 ± 3.8 | 197 ± 16 |
| Bloodstream forms | < 5 | ND | 260 ± 28 |
| pLew100–TcGLO1c | |||
| Procyclics | 23.0 ± 1.9 | 175 ± 5.6d | 387 ± 27 |
| Bloodstream forms | 38.2 ± 1.9 | ND | 810 ± 40 |
a
Values are the weighted means of three independent experiments.
b
All data represent the mean ± SD of six determinations from two independent experiments.
c
Cell lines were grown in the presence of tetracycline for 24 h prior to analysis.
d
P< 0.001 as compared to T. brucei.