Table 4.
Comparison of methylglyoxal-stimulated d-lactate and l-lactate production by wild-type and transgenic T. brucei cell lines. Data represents the mean ± SD of six determinations from two independent experiments.
| Lactate (μm) |
||||||
|---|---|---|---|---|---|---|
|
d-Lactate |
l-lactate |
|||||
| Cell line | Plus methylglyoxal | Minus methylglyoxal | Net | Plus methylglyoxal | Minus methylglyoxal | Net |
| T. brucei | ||||||
| Procyclics | 22 ± 3 | 10 ± 2 | 12 | 148 ± 7 | 24 ± 3 | 124 |
| Bloodstream forms | 68 ± 9 | 50 ± 2 | 18 | 355 ± 42 | 134 ± 18 | 221 |
| pLew100–luciferase | ||||||
| Procyclics | 17 ± 2 | 9 ± 1 | 8 | 134 ± 12 | 19 ± 2 | 115 |
| Bloodstream forms | 59 ± 2 | 48 ± 3 | 11 | 329 ± 26 | 118 ± 21 | 211 |
| pLew100–TcGLO1 | ||||||
| Procyclics | 57 ± 4a | 19 ± 2 | 38 | 122 ± 9 | 22 ± 2 | 100 |
| Bloodstream forms | 183 ± 24a | 71 ± 5 | 112 | 308 ± 14 | 105 ± 8 | 203 |
a
P< 0.001 as compared to T. brucei.