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. 2009 May;11(5):1038–1055. doi: 10.1111/j.1462-2920.2008.01825.x

Fig. 4.

Fig. 4

Genomic context of the hdrA, hdrF, hdrD and hdrL genes of Db. autotrophicum HRM2. Deduced heterodisulfide reductase genes are marked in red. The HdrA/HdrL proteins are encoded at nine genetic loci, one of which contains a tandem associated hdrL/hdrA copy (VIII). Each hdrA or hdrL locus is associated with a methylviologen non-reducing hydrogenase subunit D (mvhD), beside the hdrA4 locus, which is followed by a deduced hdrD2 gene activity (VIII). The loci V, VI, VII and VIII contain genes for a formate dehydrogenase subunit B, which is followed directly by formate dehydrogenase subunit A genes in loci V and VI. In locus VIII hdrA4 gene is associated with genes for deduced orthologues of subunits HdrB and HdrC, giving an hdrACB-like operon as can be found in Desulfovibrio species. The HdrF1 and HdrF2 genes are associated with genes for anaerobic glycerol-3-phosphate dehydrogenase activity (GlpAB) which probably transfers electrons from dicarboxylic acid degradation and genes for dicarboxylic acids transporters (Dct). hdrF3 is clustered with electron transfer flavoprotein subunits EtfBA, which might transfer electrons from the β-oxidation of fatty acids. Abbreviations are: hdrA, heterodisulfide reductase, subunit HdrA; hdrL, predicted heterodisulfide reductase/glutamate synthase fusion protein, subunit HdrL; hdrF, heterodisulfide reductase subunit HdrF; hdrD, heterodisulfide reductase, subunit HdrD (HdrCB homologous protein); dsrA, dissimilatory sulfite reductase, α-subunit; dsrB, dissimilatory sulfite reductase, β-subunit; dsrC, dissimilatory sulfite reductase, γ-subunit; mvhD, methylviologen non-reducing hydrogenase, iron-sulfur subunit MvhD; mvhG, methylviologen non-reducing hydrogenase, iron-sulfur subunit MvhG; mvhA, methylviologen non-reducing hydrogenase, nickel-iron subunit MvhA; glpA, anaerobic glycerol-3-phosphate dehydrogenase large subunit; glpB, anaerobic glycerol-3-phosphate dehydrogenase small subunit; etfA, electron transfer flavoprotein α-subunit; etfB, electron transfer flavoprotein β-subunit; dctP1 TRAP-type C4-dicarboxylate transporter, periplasmic solute-binding component; dctM1, TRAP-type C4-dicarboxylate permease, large subunit; dctQ1, TRAP-type C4-dicarboxylate permease, small subunit; 1, HysB, periplasmic [Ni/Fe/Se] hydrogenase, large subunit; 2, HysD, periplasmic [Ni/Fe/Se] hydrogenase, small subunit; 3, HyaD2, hydrogenase expression/formation protein; 4, hypothetical protein; 5, response regulator; 6, histidine kinase; 7, ferredoxin; 8, Fe/S cluster protein; 9, Aor5, tungsten-containing aldehyde reductase; 10, CheY-like regulator; 11, putative regulatory protein.