Skip to main content
. Author manuscript; available in PMC: 2009 Jun 28.
Published in final edited form as: J Neurochem. 2009 Feb 24;109(3):792–806. doi: 10.1111/j.1471-4159.2009.06012.x

Table 2.

Effects of VIP antagonist (SN)VIPhyb on VIP-promoted increase in ATP level, switch from necrosis to apoptosis, and cell attachment in oxidative stress-injured corneal endothelium

VIP VIP+VIP Antagonist P value
ATP 1.00±0.04 (N=7) 0.81±0.11 (N=7) 0.06
Apoptosis/Necrosis (%/%) (81±4)/(19±4) (N=3/N=3) (57±5)/(43±5) (N=3/N=3) 0.01/0.01
Cell Survival 1.00±0.08 (N=7) 0.78±0.05 (N=9) 0.03

ATP level was determined following the same procedure as described in Table 1. Apoptotic cells (showing peripheral chromatin condensation) and necrotic cells (showing spindle-shaped nuclei) as those depicted in Fig. 3A were counted after a 17h-exposure to H2O2 under the condition described in Fig. 3. Cell survival (attachment) was determined after 17h-exposure to H2O2 following the procedure described in Fig. 5. VIP concentration was 10−8 M in all experiments and (SN)VIPhyb was used at 5×10−7 M in experiments for ATP determination and necrosis to apoptosis switch, and at 10−6 M in the experiment for cell attachment.