TABLE 3. ¹H NMR chemical shifts of 3′-O-deacylated lipid A versus E. coli lipid A.

¹H chemical shifts at 25 °C in CDCl₃:CD₃OD:D₂O (2:3:1, v/v) relative to internal tetramethylsilane (TMS).

Position	3′-O-deacylated lipid A	E. coli lipid A
	δH, [(mult), J(Hz)]	δH, [(mult), J(Hz)]
1	5.449 [dd, 3.3, 7.0]	5.450 [dd, 3.3, 6.8]
2	4.137 [ddd, 3.3, 10.9]	4.147 [ddd, 3.3, 10.6]
3	5.192 [dd, 10.9,9.3]	5.213 [dd, 10.8,9.3]
4	3.650 [dd, 9.3]	3.679 [dd, 9.3]
5	4.031 [m]	∼4.03 [m]
6a	4.071 [m,12.0]	4.103 [dd, 2.0, 12.3]
6b	∼3.82 [m]	3.847 [dd, 5.1, 12.3]
1′	4.549 [d, 8.4]	4.613 [d, 8.8]
2′	3.674 [m]	∼3.83 [dd, 8.8, 10.8]
3′	3.745 [m]	5.18 [dd, 10.8,9.3]
4′	3.958 [m]	4.166 [dd, 9.3]
5′	3.415 [m]	3.460 [m]
6′a	∼3.82 [m]	∼3.94 [dd, 2.0, 12.3]
6′b	∼3.82 [m]	3.774 [dd, 5.1, 12.3]
α2a	2.33 [m]	∼2.33
α2b	2.29 [m]
β2	∼3.92 [m]	∼3.95 [m]
γ2	∼1.42 [m]	∼1.45 [m]
α3a	2.472 [dd, 2.3, 15.3]	∼2.45
α3b	2.423 [dd, 9.6, 15.3]
β3	∼3.98 [m]	∼4.03 [m]
γ3	∼1.45 [m]	∼1.50 [m]
α2′a	2.618 [dd, 7.4, 14.7]	∼2.561
α2′b	2.526 [dd, 6.4, 14.7]
β2′	∼5.23 [m]	∼5.18 [m]
γ2′	∼1.62 [m]	∼1.61 [m]
α3′a		2.727 [dd, 7.2, 16.3]
α3′b		2.665 [dd, 5.5, 16.3]
β3′		∼5.25 [m]
γ3′		∼1.61 [m]
α CH2	∼2.32 [m]	∼2.34 [m]
β CH2	∼1.62 [m]	∼1.61 [m]
(CH2)n	∼1.254 [m]	∼1.24 [m]
ω – 2 CH2	∼1.87 [m]	∼1.87 [m]
ω –1 CH2	1.279 [m]	1.279 [m]
ω CH3	0.886 [t, ∼7]	0.891 [t, ∼7]