A. mRNA levels, quantified by RT-PCR as described in Materials and Methods, are reported relative to wild type. Error bars represent standard deviation between biological replicates. ‘*’ denotes p < 0.05 between GK2 and wild type. Abbreviations: G6PDHx, X-linked glucose-6-phosphate dehydrogenase; F16bPase, fructose-1,6-bisphosphatase; PyK, pyruvate kinase; PEPCK, phosphoenolpyruvate carboxykinase; CS, citrate synthase.