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Nondenaturing gel electrophoresis of purified CBD-cohesin hybrids and the CelD–CBD complexes. Complexes were formed as indicated in Materials and Methods using 90 pmol of each partner. Lane 1: CBDCBH1-cohesin; lane 2: CelD–CBDCBH1 complex; lane 3: CBDCBH2-cohesin; lane 4: CelD–CBDCBH2 complex; lane 5: CBDCex-cohesin; lane 6: CelD–CBDCex complex; lane 7: CBDCipA-cohesin; lane 8: CelD–CBDCipA complex; lane 9: CelD alone.
