Fig. 4.

Identification of regions in rat GGT promoter that is required for HNE responsiveness. A series of constructs were generated by cloning different lengths of DNA fragments derived from the proximal region of GP5 into promoterless pGL-3 basic luciferase vector (A) or pGL-3 promoter luciferase vector (B). Cells were transiently transfected with reporter vectors and then treated with or without HNE. Luciferase activity was determined 24 h after HNE treatment. Transfection efficiency was controlled by normalization with cotransfected β-galactosidase. n = 3, *i < 0.01 compared with vehicle control, #p < 0.01 compared with vehicle vector.