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. Author manuscript; available in PMC: 2009 Jun 28.
Published in final edited form as: Free Radic Biol Med. 2005 Dec 1;40(8):1281–1292. doi: 10.1016/j.freeradbiomed.2005.11.005

Fig. 5.

Fig. 5

Mutation analysis of EpRE in rat GP5. (A) Nucleotide sequence of oligonucleotides used to examine EpRE activity; mutated nucleotides are underlined. (B) Mutation in GP5 EpRE core sequence abrogated its HNE responsiveness. L2 cells were transfected with luciferase reporters generated by cloning oligonucleotides containing wild-type and mutated EpREs into pGL-3 promoter luciferase vector. (C) Mutation assay at whole gene level. Site-directed mutagenesis was performed with GP5 (−645/+18)-Luc as the template, and constructs were transient-transfected into L2 cells. n = 3, *p < 0.01 compared with vehicle control, #p < 0.01 compared with vehicle vector.

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