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. Author manuscript; available in PMC: 2009 Jun 28.
Published in final edited form as: Free Radic Biol Med. 2005 Dec 1;40(8):1281–1292. doi: 10.1016/j.freeradbiomed.2005.11.005

Fig. 6.

Fig. 6

EMSA of GP5 EpRE probe with nuclear extracts from L2 cells. Nuclear extracts were prepared from L2 cells treated with/without 15 µM HNE for the indicated times. Synthetic oligonucleotides containing GP5 EpRE were annealed, end-labeled, and then incubated with nuclear extract. The DNA/protein complex was analyzed with EMSA. Representative image was generated by the Cyclone Storage Phosphor System from one typical experiment (top) and the semiquantitative graphical summary (bottom) was based on radioactivity counts generated by OptiQuant image analysis software. n = 3, *p < 0.01 compared with vehicle control; #p < 0.01 compared with the intensity of EpRE complex of 1 h after HNE treatment. N, nuclear factor κB oligonucleotides; C, cold GP5 EpRE; M1 and M2, mutation forms of GP5 EpRE.

HHS Vulnerability Disclosure