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. 2009 Jul 9;4(7):e6186. doi: 10.1371/journal.pone.0006186

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Jagarlamudi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Morphological analysis of ovaries from 13- and 23-day-old, and 16-week-old Pten^loxP/loxP; Zp3-Cre+ mice, Pten^loxP/loxP; GCre+ mice, and control Pten^loxP/loxP mice. Ovaries were embedded in paraffin and sections of 8-µm thickness were prepared and stained with hematoxylin. Note the overactivation of primordial follicles in Pten^loxP/loxP; GCre+ ovaries (C, F, and I, arrows) and the normal follicular development and CL in Pten^loxP/loxP; Zp3-Cre+ ovaries (B, E, H and K), which is comparable to the control Pten^loxP/loxP ovaries (A, D, G, and J). CL, corpora lutea.