Figure 5. Normal GVBD, ovulation, fertilization, and fertility in Pten^loxP/loxP; Zp3-Cre+ mice.

(A) Oocytes from 3–4-week-old PMSG-primed Pten^loxP/loxP; Zp3-Cre+ mice or control Pten^loxP/loxP mice were collected in M2 medium. They were cultured further in M16 medium for GVBD analysis. GVBD rate was scored after a culture period of 6 h. Normal GVBD rates were observed in Pten mutant oocytes as compared to control oocytes. The numbers of oocytes used (n) are shown. (B) Two-cell embryos were collected at E1.5 and were cultured in KSOM medium supplemented with amino acids. Numbers of 2-cell embryos per female (mean±SEM) mouse were counted. The numbers of mice used (n) and the results of statistical analysis are shown. No significant difference (NS) was seen between Pten^loxP/loxP; Zp3-Cre+ mice and control mice. (C) Normal litter size of Pten^loxP/loxP; Zp3-Cre+ females as compared to Pten^loxP/loxP control mice (mean±SEM). The numbers of litters (n) from 10 mice of each genotype and results of statistical analysis are shown. NS, not statistically significant.